Figure 3.

Collision response as a function of TGFβ treatment. (A) Fraction sliding and (B) CFD for TGFβ-treated and untreated (control, square) cells. MCF-10A cells were treated with either 5 ng/mL TGFβ (triangle) for three days (solid representation, solid line) or six days (open, dashed line); and 20 ng/mL TGFβ (circle) for three days (solid representation, solid line) or 12 days (open, dashed line). In total, 7226 cell-cell collisions were analyzed across all 50 combinations of five TGFβ treatment conditions and 10 micropattern widths (detailed breakdown of sample sizes provided in Table S2). For each TGFβ treatment condition, including control, the dependence of fraction sliding on line width is statistically significant (p < 0.001). Meanwhile, the dependence of fraction sliding on TGFβ treatment was statistically significant at line widths of 12 μm (p < 0.001) and 9, 15, 21, 27, and 33 μm (p < 0.05). The dependence of fraction sliding on TGFβ treatment at line widths of 6, 18, 24, and 30 μm were not statistically significant. Further details on analysis of variance are provided in the Supporting Material. Error bars denote mean ± SE.