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. 2016 Oct 6;6:34784. doi: 10.1038/srep34784

Figure 2. MB affects PAR1 function and protein level at NMJ in PAR1 OE larvae.

Figure 2

(a) High-magnification views of the distribution patterns of Dlg at larval NMJ. NMJs of vehicle and drug treated PAR1 OE larvae were double-labeled with anti-Dlg (red) and anti-HRP (green) antibodies. Scale bar = 2 μm. PAR1 OE larvae were fed with vehicle or drugs as indicated. (b) MB reduces PAR1 protein level in larval muscle walls. Muscle walls were dissected out from late third instar larvae and homogenized for WB analysis. Representative immunoblot showing PAR1 protein level with β-actin as loading control. (c) Quantification of PAR1 level normalized to β-actin presented as percent of vehicle treated control (n = 3). **0.001 < P < 0.01.