Table 1. Strains and plasmids.

Strain or plasmid	Relevant properties	Reference or source
Plasmids
pTA131	pBluescript II with BamHI-Xbal I fragment from pGB70 containing pfdx-pyrE2; bla (AmpR)	31
pIDJL40	gfp-fusion expression vector derived from pTA962	39, 40
pJWID1	Shuttle vector derived from pTA962 with pyrE2, hdrB, hmgA, bla, SmRS-GFP and pHV2 origin of replication	This study
pTA131_Δamd3	pTA131 with EcoRI-NotI fragment containing hmgA and amd3 flanking regions for gene inactivation	This study
pJWID1_amd3	pJWID1 with NdeI-EcoRI fragment containing amd3	This study
Strains
E. coli c2925	dam, dcm strain; used for preparing unmethylated plasmid DNA	New England Biolabs, C2925I
Hfx. volcanii DS2	Source of hmgA gene	33
Hrr. lacusprofundi ACAM34	Wild-type	7
Hrr. lacusprofundi Δamd3	Hrr. lacusprofundi ACAM34 with amd3 gene disruption	This study