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. 2016 Oct 6;6:34528. doi: 10.1038/srep34528

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Dendritic segments of hippocampal neurons (21 DIV) transfected with extracellularly HA-tagged Ca_V1.3_L or Ca_V1.3_ΔITTL without or with densin-180 (+densin). In conditions with elongated dendritic spines (Ca_V1.3_L + densin-180, Ca_V1.3_ΔITTL, Ca_V1.3_ΔITTL + densin-180) Ca_V1.3 channel clusters are more often found within the neck region of dendritic spines and in the spine head (examples indicated by arrowheads). (b) Heatmap graphs showing the localization probability of Ca_V1.3 channel clusters along the dendritic spine (y-axis, distance from dendrite) and laterally in relation to the main axis of the spine (x-axis, distance from spine). Negative y-values (below the 0 line) represent channel clusters positioned at the spine base. The fraction of channel clusters >1 μm from the spine base (red values) was significantly increased in the Ca_V1.3_L + densin-180, Ca_V1.3_ΔITTL, and Ca_V1.3_ΔITTL + densin-180 conditions (χ² test, p = 0.04). (c) Cumulative frequency distribution of cluster distances from the spine base (upper panel; only positive values included) and fractional difference from the mean distance of control (Ca_V1.3_L) further demonstrate a redistribution of Ca_V1.3 channel clusters along the elongated dendritic spines. Statistics: b: Chi-square test: χ²₍₉₎ = 17.6, p = 0.04; c: ANOVA with Holm-Sidak posthoc analysis was performed on the log10-transformed raw data (F_3,978 = 5.1, p = 0.002; posthoc: *p = 0.012, **p < 0.01). Scale bar, 5 μm.