Skip to main content
. 2016 Oct 6;6:34543. doi: 10.1038/srep34543

Figure 3. c-Jun is required for ERK-dependent DNA demethylation of Zta promoter.

Figure 3

(a) B95-8 cells were lentiviruses expressing control shRNA (shCtrl) or shRNA against c-Jun (shc-Jun) for 2 weeks and then selected with puromycin. The total RNAs were isolated and the c-Jun mRNA was analyzed by qPCR (a). (b–d) B95-8 cells were transduced with lentiviruses expressing shCtrl or shc-Jun for 2 weeks, selected with puromycin, and then treated with TPA for 18 h. The total RNAs were isolated form the cells and the level of Zta mRNA was determined by qPCR (b). The cell extracts were prepared and the level of Zta, c-Jun, and β-actin proteins were detected by Western blot analyses (c). The Zta promoter was analyzed by sodium bisulphite sequencing. The white and black circles indicate unmethylated and methylated CpGs, respectively (d). All data were collected from 3 independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.