Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 6;6:34808. doi: 10.1038/srep34808

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

25-mer Arrays were assembled with either unmodified histones (WT) or H3 6KQ/H4 4KQ in the central DC nucleosome. (A) Arrays are not self-associated in digestion buffer containing 0.5 mM magnesium acetate. DraIII digestion buffer was prepared containing either 0.5 mM or 10 mM magnesium acetate (lanes 2 and 5 or 3 and 6, respectively) and the extent of self-association determined by a centrifuge assay. Arrays in TE buffer are shown for comparison (lanes 1 and 4). (B) Effect of reduced self-association on DC nucleosome linker DNA accessibility. The relative rate (krel) of DraIII digestion in buffer containing 0.5 mM magnesium acetate was determined. krels are indicated below the gels. (C) DC nucleosomes were ligated with naked p12 templates and the relative rate of digestion in NEB4 buffer determined. Lanes 1–10 show digestion time points taken over 60 minutes, as indicated.