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. 2016 Oct 6;6:34909. doi: 10.1038/srep34909

Figure 2. Effect of intracarotid BHB infusion on orexigenic and anorexigenic hypothalamic neuropeptide mRNA expression as well as on the mRNA expression of ketogenic enzymes in the hypothalamus and in the liver.

Figure 2

(a) Hypothalamic NPY mRNA expression at 24 h in mice infused with either NaCl or BHB (b) Hypothalamic AgRP mRNA expression at 24 h in mice infused with either NaCl or BHB (c) Hypothalamic POMC mRNA expression at 24 h in mice infused with either NaCl or BHB (d) Hypothalamic CART mRNA expression at 24 h in mice infused with either NaCl or BHB. (e) Hypothalamic HMG-CoAs mRNA expression at 24 h in mice infused with either NaCl or BHB. (f) Hypothalamic BHBDH mRNA expression at 24 h in mice infused with either NaCl or BHB. (g) Liver HMG-CoAs mRNA expression at 24 h in mice infused with either NaCl or BHB. (h) Liver BHBDH mRNA expression at 24 h in mice infused with either NaCl or BHB. mRNA expression for each hypothalamic peptide and enzyme involved in ketone bodies metabolism was determined by quantitative reverse transcriptase–PCR at 24 h of NaCl or BHB infusion. Data represent the mean ± SEM with n = 9–12 animals per condition and were statistically analyzed with unpaired Student t-test. *p < 0.05 vs. NaCl; **p < 0.01 vs. NaCl. a.u., arbitrary units.