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. 2016 Oct 6;6:34854. doi: 10.1038/srep34854

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Flow diagram used to investigate the inositol deacylation of GPI anchored protein (Als1p) in C. albicans assessed by its sensitivity to bacterial phosphatidylinositol-specific phospholipase C (PI-PLC). (B) Sensitivity of Als1p from parent and bst1Δ/Δ mutant strains to PI-PLC. Purified HA-tagged Als1p were treated as specified in Fig. 1A. After phase separation, both aqueous (A) and detergent (D) phases of Als1p underwent immunoblotting using anti-HA tag antibody. (C) Sensitivity of mannoproteins from parent SN152, BST1-complemented, bst1Δ/Δ null mutant and BST1S202A mutant strains to PI-PLC. Cytoplasmic proteins from the detergent (D) phases were treated as specified in Fig. 1A. After phase separation, both aqueous (A) and detergent (D) phases of proteins underwent immunoblotting using 2.5 μg/ml peroxidase labeled-Con.