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. 2016 Sep 27;7:12874. doi: 10.1038/ncomms12874

Figure 2. CARD9 modulates pro-IL-1β expression.

Figure 2

(a–f) Quantitative PCR analysis of WT and Card9−/− BMDMs infected with S. Typhimurium (m.o.i. 5) for 2 h, compared with uninfected controls. (a) Pro-IL-1β, (b) NLRP3, (c) TNF-α, (d) RANTES, (e) caspase-8 and (f) SYK. (g) Expression of pro-IL-1β, SYK, pro-caspase-8, pro-caspase-1, CARD9, ASC, β-actin in cell lysates, and caspase-1 p10, caspase-8 p18 and IL-1β in culture supernatants from WT, Card9−/− and Pycard−/− BMDMs after 2 or 6 h of infection with S. Typhimurium (m.o.i. 5) and relative densitometry (Card9−/−/WT) in culture supernatants (h) or cell lysates (Card9−/−/WT, actin-normalized) (i). *P<0.05 in comparison with uninfected control, unless stated in the graph (one-way analysis of variance with Tukey's multiple comparisons test). (a–f,h,i) Data from three independent experiments (mean and s.e.m.). (g) Image is representative of three independent experiments.