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. 2016 Sep 27;7:12874. doi: 10.1038/ncomms12874

Figure 4. CARD9 selectively negatively regulates NLRP3-induced IL-1β production.

Figure 4

(a,b,e,f) Cellular viability and (c,d,g,h) IL-1β secretion from unprimed WT and Card9−/− BMDMs infected with S. Typhimurium at m.o.i. 10 for 2 (a,c,e,g) and 6 (b,d,f,h) h in presence or absence of NLRP3 inhibitors glibenclamide (a–d) and MCC950 (e–h). (i,j) Cellular viability (i) and IL-1β secretion (j) from LPS-primed BMDMs after nigericin stimulation (10 μM, 1 h). IL-1β from LPS-primed cells without nigericin stimulation was below the level of detection. (k,l) Cellular viability (k) and IL-1β secretion (l) from LPS-primed BMDMs after ATP stimulation (5 mM, 30 min). *P<0.05 in comparison with WT (one-way analysis of variance with Tukey's multiple comparisons test). (a–l) Data from three independent experiments (mean and s.e.m.).