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. 2016 Sep 27;7:12874. doi: 10.1038/ncomms12874

Figure 6. SYK and caspase-8 activity are important for CARD9 regulation of NLRP3 activity in BMDMs, but not in BMDCs.

Figure 6

(a–d) Effects of caspase-8 inhibition by Z-IETD-FMK on LPS-primed WT and Card9−/− LPS-primed BMDMs on cellular viability (a,c) and IL-1β production (b,d) during S. Typhimurium infection (m.o.i. 10, 1 h) (a,b) or nigericin (10 μM, 1 h) stimulation (c,d). (e–h) Effects of SYK inhibition by R406 on WT and Card9−/− LPS-primed BMDMs on cellular viability (e,g) and IL-1β secretion (f,h) in response to S. Typhimurium infection (m.o.i. 10, 1 h) (e,f) or nigericin stimulation (5 μM, 1 h) (g,h). (i,j) Stimulation of WT or Card9−/− BMDCs with nigericin (5 μM, 1 h). Cellular viability (i) and IL-1β secretion (j). *P<0.05 (one-way analysis of variance with Tukey's multiple comparisons test. (a–j) Data from three independent experiments (mean and s.e.m.).