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. 2016 Sep 27;7:12874. doi: 10.1038/ncomms12874

Figure 7. SYK activity increases the number of caspase-8 specks in response to NLRP3 stimulation in Card9−/− BMDMs.

Figure 7

(a–c) WT, Card9−/− or Pycard−/− LPS-primed BMDMs were incubated with nigericin (5 μM, 30 min) in the presence of caspase-1 and caspase-8 FLICA substrates, with or without the SYK inhibitor R406. (a) Pattern of caspase-1 and caspase-8 specks observed by immunofluorescence labelling of the protein. Percentage of cells containing (b) caspase-8 or (c) caspase-1 specks. *P<0.05 (one-way analysis of variance with Tukey's multiple comparisons test). (a) Image is representative of three independent experiments. (b,c) Data from three independent experiments (mean and s.e.m.). Scale bar, 10 μm.