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. 2016 Oct 6;6:34496. doi: 10.1038/srep34496

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Hep G2 cells were transfected with HBx and were incubated with HA (50 or 100 μM). Western blots were performed for C-myc and SIRT-1 from total cellular protein. Lane 1, control; lane 2, empty vector transfected cells; lane 3, HBx transfected cells; lane 4, HBx transfected and HA (50 μM) incubated cells; and lane 5, HBx transfected and HA (100 μM) incubated cells. Empty vector was used as transfection control in all the experiments. β-actin was used as an internal control (n = 3). (B) The band intensities were quantified using Image J software and the average from 3 experiments are plotted as fold change (*p < 0.05 compared to control; ^#p < 0.05 compared to HBx transfected cells).