Table 1.
Antibody list and surface stain panel.
| Antibody | Conjugate | Clone | Specificity | Concentration | Company |
|---|---|---|---|---|---|
| Common monocyte/dendritic cell surface markers | |||||
| Dead Cell Stain | Aqua | N/A | Dead cells | 0.2 μl/test | Invitrogen |
| CD3ε | Biotin | 145-2C11 | T cells | 5 μg/ml | Biolegend |
| CD19 | Biotin | 6D5 | B cells | 5 μg/ml | Biolegend |
| Ly6G | Biotin | 1A8 | Neutrophils | 5 μg/ml | Biolegend |
| NKp46 | Biotin | 29A1.4 | NK cells | 10 μg/ml | Biolegend |
| CD11b | APC-Cy7 | M1/70 | CD11b | 1 μg/ml | Biolegend |
| CD11c | PE-Cy7 | N418 | CD11c | 1 μg/ml | Biolegend |
| Ly6C | PB | HK1.4 | Ly6C | 2.5 μg/ml | Biolegend |
| Streptavidin | BV510 | N/A | Biotin | 2 μg/ml | BD Bioscience |
| Live monocytic/dendritic cell panel | |||||
| Siglec-H | APC | 551 | pDCs | 2 μg/ml | Biolegend |
| CD4 | PerCP-Cy5.5 | GK1.5 | CD4 | 1 μg/ml | Biolegend |
| CD8α | BV605 | 53–6.7 | CD8α | 1 μg/ml | Biolegend |
| DCIR2 | PE | 33D1 | CD11b+ DCs | 2 μg/ml | Biolegend |
| RT1B | FiTC | OX-6 | MHCII/IAg7 | 2.5 μg/ml | Biolegend |
| Live pDC panel | |||||
| Siglec-H | APC | 551 | pDC marker | 2 μg/ml | Biolegend |
| CD8α | PerCP Cy5.5 | 53–6.7 | CD8α | 1 μg/ml | Biolegend |
| RT1B | FiTC | OX-6 | MHCII/IAg7 | 2.5 μg/ml | Biolegend |
| BST2 | PE | 927 | BST2/PDCA-1 | 2 μg/ml | Biolegend |
| B220 | BV605 | RA3-6B2 | CD45R/B220 | 1 μg/ml | Biolegend |
| Functional monocytic/dendritic cell panel | |||||
| Siglec-H | PerCP-Cy5.5 | 551 | pDCs | 2 μg/ml | Biolegend |
| CD8α | BV605 | 53–6.7 | CD8α | 1 μg/ml | Biolegend |
| DCIR2 | FITC | 33D1 | CD11b+ DCs | 5 μg/ml | Biolegend |
| RT1B | AF647 | OX-6 | MHCII/IAg7 | 1 μg/ml | BD Bioscience |
| CD206 | PE | C068C2 | Mannose receptor | 2 μg/ml | Biolegend |
| CD209 | PE | 5H10 | DC-SIGN | 2 μg/ml | eBioscience |