Table 3.
LSRII Dector Configuration | |||
---|---|---|---|
Laser | Fluorochrome Detected | Dichroic LP Filter | BP Filter |
Blue 488 nm | Forward Scatter | ||
Blue 488 nm | PerCP Cy5.5, PerCP | 685LP | 710/50 |
Blue 488 nm | FITC, CFSE, GFP, Alexa 488, GrViD, Cy2 | 505LP | 515/20 |
Blue 488 nm | Side Scatter | 488LP | |
Green 532 nm | PE Cy7, Alexa 750 | 740LP | 780/40 |
Green 532 nm | PE, Cy3, Alexa 532 | empty | 575/25 |
Red 633 nm | APC Cy7, Alexa 750 | 740LP | 780/60 |
Red 633 nm | APC, Alexa 647 | empty | 660/20 |
Violet 407 nm | QD605 | 595LP | 605/40 |
Violet 407 nm | QD545, Pacific Orange, Aqua | 535LP | 560/40 |
Violet 407 nm | Cblue, ViViD, Pacific Blue | empty | 450/50 |