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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: J Immunol Methods. 2015 Sep 5;432:4–12. doi: 10.1016/j.jim.2015.08.015

Table 3.

LSRII configuration.

LSRII Dector Configuration
Laser Fluorochrome Detected Dichroic LP Filter BP Filter
Blue 488 nm Forward Scatter
Blue 488 nm PerCP Cy5.5, PerCP 685LP 710/50
Blue 488 nm FITC, CFSE, GFP, Alexa 488, GrViD, Cy2 505LP 515/20
Blue 488 nm Side Scatter 488LP
Green 532 nm PE Cy7, Alexa 750 740LP 780/40
Green 532 nm PE, Cy3, Alexa 532 empty 575/25
Red 633 nm APC Cy7, Alexa 750 740LP 780/60
Red 633 nm APC, Alexa 647 empty 660/20
Violet 407 nm QD605 595LP 605/40
Violet 407 nm QD545, Pacific Orange, Aqua 535LP 560/40
Violet 407 nm Cblue, ViViD, Pacific Blue empty 450/50