Figure 2. Knockdown of SOX12 in breast cancer cells. (A) The mRNA expression levels of SOX12 in six human breast cancer cells were determined by real-time PCR and normalized with GAPDH. (B) The protein levels of SOX12 were determined in human breast cancer cells by Western blot. GAPDH was used as the loading control. (C) Knockdown of SOX12 in BT474 and MCF-7 cells detected by real-time PCR. (D) Knockdown of SOX12 in BT474 and MCF-7 cells detected by Western blot. Upper panels: representative images; lower panels: quantifications of Western blot. WT: wild-type cells; shNC: cells infected with scrambled shRNA virus; shSOX12-1: cells infected with shSOX12-1 virus; shSOX12-2: cells infected with shSOX12 virus. **P<0.01 and ***P<0.001 compared with shNC.
