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. 2016 Nov 5;371(1707):20150492. doi: 10.1098/rstb.2015.0492

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 6. — (a) Fluorescence images in false colours of electrocompetent E. coli cells electroporated with 1.5 µM (30 pmol) CheY(Cys)-Cy3B imaged in DIC, fluorescence and an overlap of the two; (b) electrocompetent cells incubated with the same amount of protein but not electroporated showed little or no fluorescence internalization. (c) Detail showing polar localization of electroporated CheY-Cy3B proteins inside the cells; (d) Further control: electroporated free Cy3B-maleimide dye shows no specific localization. Epifluorescence, 150 ms exposure time. Electroporation parameters and procedure as described in §2c.