Table 2.
Recommended labels for different techniques. ++, very good; +, good; (+), possible, but not demonstrated yet; —, poor/not applicable; FP, fluorescent protein; pa-FP, photoactivatable FP; ps-FP, photoswitchable FP; pc-FP photoconvertible FP; TC-tag, tetracystein-tag; UAA, unnatural amino acid incorporation; FbFPs, flavin binding fluorescent proteins; FA-tag, fluorescence-activating tag. For references see text.
| diffraction limited |
super-resolution |
|||
|---|---|---|---|---|
| STED | SIM | PALM/STORM | ||
| ‘conventional’ FPs | ++ | ++b | ++b | — |
| pa-FPs/ps-FPs/pc-FPs | + | + | +c | ++ |
| SNAP-/ CLIP-/HALO-taga | ++ | ++ | ++ | ++ |
| TC-tag | ++ | (+) | (+) | + |
| UAAa | ++ | ++ | ++ | ++ |
| FbFPs | + | — | (+) | + |
| FA-tag | ++ | (+) | (+) | (+) |
aOnly in combination with small organic dyes.
bHigh fluorophore stability and brightness required.
cOnly with pc-FPs.