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. 2016 Nov 5;371(1707):20150505. doi: 10.1098/rstb.2015.0505

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — Replication analysis of the different SaPIbov5 derivative islands. Southern blot of ϕ12 and ϕSLT lysates, from strains carrying SaPIbov5^original, SaPIbov5^adjusted and SaPIbov5^evolved as indicated (see text for details). Samples were isolated 0 or 90 min after induction with mitomycin C, separated on agarose gels and blotted with a SaPIbov5-specific probe. Upper band is ‘bulk’ DNA, and represents replicating SaPIbov5. SaPI monomer represents SaPI DNA packaged in small capsids.