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. 2016 Nov 5;371(1707):20150505. doi: 10.1098/rstb.2015.0505

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© 2016 The Authors.

Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 5. — SaPIbov5 Ccm-mediated interference. (a) Strain RN4220 containing wt or the different SaPIbov5 mutants were infected with ϕ12 or ϕ12^evolved4, plated on phage bottom agar, and incubated for 48 h at 32°C. (b) Phage interference mediated by cloned SaPIbov5 genes. The indicated genes were cloned into plasmid pCN51. Strain RN4220 containing the indicated plasmids was infected with phages 12 or ϕ12^evolved4, plated on phage bottom agar containing 5 µM CdCl2 (induces the expression of the cloned genes) and incubated for 48 h at 32°C. (c) Effect of the different pCN51 cloned genes in phage reproduction. The lysogenic strains for ϕ12 or ϕ12^evolved4, containing the different pCN51 derivative plasmids, were SOS induced and the lysates plated on phage bottom agar for 48 h at 32°C.