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. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: Biochem J. 2015 Mar 1;466(2):311–322. doi: 10.1042/BJ20141373

Figure 4. Effects of phosphorylation on SRSF1 and SR(3SAP) binding to the Ron ESE.

Figure 4

AC) Fraction bound of SRSF1 (●) and SR(3SAP) (▲) without kinase (A) and with SRPK1 (B) or CLK1 (C) pre-treatment. Fraction bound (FB) is plotted against total SR protein concentration ([SR]) according to the equation FB = [SR]N/(Kd+[SR]N) = [SR]N/((K0.5)N+[SR]N) where Kd, K0.5 and N are the dissociation constant, concentration of half maximal saturation and the cooperativity constant for the SR protein, respectively. D) The degrees of cooperativity (N) for SRSF1 and SR(3SAP) in the absence and presence of kinase phosphorylation are plotted in the bar graph. Error bars represent standard errors from data fitting.