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GOLPH3 knockdown promoted the apoptosis of U251 and U87 cells. (A) The silencing efficacy of GOLPH3 siRNA was detected by Western blot analysis. β-actin was the loading control. (B) Quantitative analysis of GOLPH3 levels after GOLPH3 knockdown. (C, D) Flow cytometry assay showed the apoptosis rate of U251 and U87 cells at 48 h after transfection with GOLPH3 siRNA. R5 quadrant was calculated as the percentage of apoptosis rate. Data are shown as mean ±SEM (n=3). * P<0.05, ** P<0.01.
