| Data-collection parameters |
| Instrument |
BM29, ESRF |
| Wavelength (Å) |
0.99 |
|
q-range (Å−1) |
0.0032–0.49 |
| Sample-to-detector distance (m) |
2.864 |
| Exposure time per frame (s) |
1 |
| Concentration range |
n.a. |
| Temperature (K) |
293 |
| Detector |
PILATUS 1M (Dectris) |
| Flux (photons s−1) |
5 × 1011
|
| Beam size (µm) |
700 × 700 |
| Structural parameters for BSA, linear gradient |
|
|
I
0 (from Guinier) (arbitrary units) |
0.0778 |
|
R
g (from Guinier) (Å) |
27.4 ± 0.1 |
|
q
min
R
g − q
max
R
g used for Guinier |
0.31–1.28 |
| Theoretical R
g (from CRYSOL) (Å) |
27.15 |
| Porod volume V
p (from SCÅTTER) (Å3) |
(116 ± 5) × 103
|
| Molecular mass M
r (from V
p) (kDa) |
68.2 |
| Calculated monomeric M
r from sequence (kDa) |
66.5 |
| Structural parameters for BSA, step gradient |
|
I
0 (from Guinier) (arbitrary units) |
0.0183 |
|
R
g (from Guinier) (Å) |
27.1 ± 0.1 |
|
q
min
R
g − q
max
R
g used for Guinier |
0.27–1.29 |
| Theoretical R
g (from CRYSOL) (Å) |
27.15 |
| Porod volume V
p (from SCÅTTER) (Å3) |
(116 ± 5) × 103
|
| Molecular mass M
r (from V
p) (kDa) |
68.2 |
| Calculated monomeric M
r from sequence (kDa) |
66.5 |
| Structural parameters for D5323–785, step gradient |
|
I
0 (from Guinier) (arbitrary units) |
0.386 |
|
R
g (from Guinier) (Å) |
46.5 ± 0.1 |
|
q
min
R
g − q
max
R
g used for Guinier |
0.44–1.29 |
|
I
0 [from P(r)] |
0.378 |
|
R
g [from P(r)] (Å) |
44.7 ± 0.1 |
|
D
max [from P(r)] (Å) |
120 |
|
q-range used for P(r) (Å−1) |
0.03–0.18 |
| Porod volume V
p (from SCÅTTER) (Å3) |
(577 ± 5) × 103
|
| Molecular mass M
r (from V
p) (kDa) |
338 |
| Calculated monomeric M
r from sequence (kDa) |
321 |
