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. 2016 Oct 6;1(16):e89371. doi: 10.1172/jci.insight.89371

Figure 1. Effect of furin inhibition on the secretion and intracellular retention of human factor VIII (hFVIII) variants.

Figure 1

The amount of hFVIII secreted (A and C) or intracellularly retained (B and D) from baby hamster kidney (BHK) cells stably expressing either hFVIII-BDD (A and B) or hFVIII-ΔF (C and D) after transduction with vectors containing either the furin-inhibiting α1-antitrypsin Portland (A1AT-PDX) or a negative control (A1AT-wild type [A1AT-WT] or EGFP). Intracellular FVIII is reported as the relative amount of FVIII after transduction compared with the mean of the EGFP-transduced group. (E) Direct comparison of the amount of secreted (left) and intracellularly retained (right) hFVIII in BHK cells expressing either hFVIII-BDD or hFVIII-ΔF. All FVIII levels were determined by ELISA in duplicate. Each data point represents a distinct transduction and results are aggregates of 2–4 distinct experiments. Means were compared by 2-tailed Student’s t test. P values greater than 0.05 were considered not significant (n.s.). Horizontal markers in whisker plots represent the mean and 1 SEM.