Figure 4.

Self-rotation function of BIK1 X-ray diffraction data. The stereographic projection from POLARRFN in the CCP4 suite (Winn et al., 2011 ▸) corresponding to a rotation angle of κ = 180° is shown. The monoclinic crystallographic twofold b axis is vertical (c* × a on the plot). A strong noncrystallographic twofold axis is also observed rotated ∼18° from the monoclinic a axis (horizontal) perpendicular to the b axis. The rotation function was calculated using all data to a resolution of 2.35 Å and a radius of integration of 20 Å. The rotation-function map was contoured starting at the level of two root-mean-square (r.m.s.) deviations, with a contour interval at every one r.m.s. deviation of the calculated map.