Table 2. Crystallization.
Method | Microbatch under oil |
Plate type | 72-well |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 3.3 |
Buffer composition of protein solution | 10 mM HEPES, 150 mM NaCl, 2 mM β-mercaptoethanol pH 7.2 |
Composition of reservoir solution | 245 mM ammonium tartrate, 12.5% PEG 3350 pH 7.0 |
Volume and ratio of drop | 2 µl final volume, 1:1 ratio of protein:reservoir |