Table 1. Mouse GPx4 production information.

Source organism	Mus musculus (mouse), cytoplasmic isoform O70325-2
DNA source	ATG:biosynthetics GmbH
Forward primer	N/A
Reverse primer	N/A
Cloning vector	pUC57
Expression vector	pETM-11
Expression host	E. coli BL21 (DE3)
Complete amino-acid sequence of the construct produced†	MGCASRDDWRCARSMHEFSAKDIDGHMVCLDKYRGFVCIVTNVASQCGKTDVNYTQLVDLHARYAECGLRILAFPCNQFGRQEPGSNQEIKEFAAGYNVKFDMYSKICVNGDDAHPLWKWMKVQPKGRGMLGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL

^†In this construct, selenocysteine 46 (underlined) was mutated to cysteine. The glycine residue in italics is a cloning artefact.