| Method |
Sitting-drop vapour diffusion |
| Plate type |
Art Robbins Instruments Intelli-Plate 96-2 low profile |
| Temperature (K) |
291 |
| Protein concentration (mg ml−1) |
11.0 |
| Buffer composition of protein solution |
50 mM Tris pH 7.5, 100 mM NaCl |
| Composition of reservoir solution |
0.1 M HEPES pH 7.5, 35%(v/v) Jeffamine ED-2001 pH 7.0, 2.0 M sodium thiocyanate |
| Volume and ratio of drop |
1 µl protein solution + 1 µl reservoir solution |
| Volume of reservoir (µl) |
100 |
| Cryoprotection solution |
30%(v/v) glycerol, 0.1 M HEPES pH 7.5, 35%(v/v) Jeffamine ED-2001 pH 7.0 |
