Table 1. Macromolecule-production information.

Macromolecule	TthTtuA	TthTtuB (G65C)
Source organism	T. thermophilus
DNA source	UniProt Q72LF3	UniProt Q72LF4
Expression vector	pCDFDuet-1	Modified pET-28a
Expression host	E. coli B834 (DE3), co-expression
Complete amino-acid sequence of the construct produced†	MVCKVCGQKAQVEMRSRGLALCREHYLDWFVKETERAIRRHRMLLPGERVLVAVSGGKDSLALWDVLSRLGYQAVGLHIELGIGEYSKRSLEVTQAFARERGLELLVVDLKEAYGFGVPELARLSGRVACSACGLSKRYIINQVAVEEGFRVVATGHNLDDEAAVLFGNLLNPQEETLSRQGPVLPEKPGLAARVKPFYRFSEREVLSYTLLRGIRYLHEECPNAKGAKSLLYKEALNLVERSMPGAKLRFLDGFLEKIRPRLDVGEEVALRECERCGYPTTGAVCAFCRMWDAVYRRAKKRKLLPEEVSFRPRVKPLRAG	MGSSHHHHHHSSGLVPRGSHMRVVLRLPERKEVEVKGNRPLREVLEELGLNPETVVAVRGEELLTLEDEVREEDTLEVLSAISGC

^†The artifactual sequences, i.e. His₆ tag, TEV protease recognition site (LVPRGS) and point mutation G65C, are underlined.