Table 1. Macromolecule-production information.
Source organism | A. thaliana | C. sativa |
DNA source | The Arabidopsis Information Resource (TAIR) | DNA synthesis |
Forward primer | 5′-GCGGATCCCACAAGCTTTGTCTATGAAAGCTTCCT-3′† | 5′-CATGGCGGCCGCAGTCAACAAGAGACAAGGTTTGTCTA-3′‡ |
Reverse primer | 5′-CATGGCGGCCGCCTAGTGGTGATGGTGGTGGTGAGGAACTTCAGGAAGTTTTGAGATATC-3′‡ | 5′-GCGGATCCCTAGTGGTGATGGTGGTGGTGTGGATGAGGAACTTTAGGAAGTTTTG-3′† |
Cloning vector | pFastBac1 | pFastBac1 |
Expression vector | Baculovirus | Baculovirus |
Expression host | Insect cells (High Five) | Insect cells (High Five) |
Complete amino-acid sequence of the construct produced§ | ADPTSFVYESFLDRQNLYLDKSAIVLPSGLLQLTNASEHQMGHAFHKKPIEFSSSGPLSFSTHFVCALVPKPGFEGGHGIVFVLSPSMDFTHAESTRYLGIFNASTNGSSSYHVLAVELDTIWNPDFKDIDHNHVGIDVNSPISVAIASASYYSDMKGSNESINLLSGNPIQVWVDYEGTLLNVSVAPLEVQKPTRPLLSHPINLTELFPNRSSLFAGFSAATGTAISDQYILWWSFSIDRGSLQRLDISKLPEVPHHHHHH | ADGGRSQQETRFVYESFLDQENLYIDKSATVLPSGILRLTNASEHQMGHAFHKKPLEFSSSGPLSFSTHFVCALVPKPRVEGGHGIAFVLSPSMDFTHAESTRYLGIFNASTSGSSSYHVLAVELDTIWNPDFKDIDHNHVGIDVNSPISVAIASASYFSDIKGSHERVDLLSGRPIQVWVDYEGTMLNVSIAPLKVQKPSRPLLSHPINLSKFFPNRSRLFVGFSASTGTAISDQYILWWSFSTRRGSLQGFDISKLPKVPHPHHHHHH |
Contains a BamHI site.
Contains a NotI site.
The sequences derived from the cloning vector and the engineered C-terminal six-histidine tags are underlined.