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. 2016 Sep 22;72(Pt 10):782–787. doi: 10.1107/S2053230X16014278

Table 1. Macromolecule-production information.

Source organism A. thaliana C. sativa
DNA source The Arabidopsis Information Resource (TAIR) DNA synthesis
Forward primer 5′-GCGGATCCCACAAGCTTTGTCTATGAAAGCTTCCT-3′ 5′-CATGGCGGCCGCAGTCAACAAGAGACAAGGTTTGTCTA-3′
Reverse primer 5′-CATGGCGGCCGCCTAGTGGTGATGGTGGTGGTGAGGAACTTCAGGAAGTTTTGAGATATC-3′ 5′-GCGGATCCCTAGTGGTGATGGTGGTGGTGTGGATGAGGAACTTTAGGAAGTTTTG-3′
Cloning vector pFastBac1 pFastBac1
Expression vector Baculovirus Baculovirus
Expression host Insect cells (High Five) Insect cells (High Five)
Complete amino-acid sequence of the construct produced§ ADPTSFVYESFLDRQNLYLDKSAIVLPSGLLQLTNASEHQMGHAFHKKPIEFSSSGPLSFSTHFVCALVPKPGFEGGHGIVFVLSPSMDFTHAESTRYLGIFNASTNGSSSYHVLAVELDTIWNPDFKDIDHNHVGIDVNSPISVAIASASYYSDMKGSNESINLLSGNPIQVWVDYEGTLLNVSVAPLEVQKPTRPLLSHPINLTELFPNRSSLFAGFSAATGTAISDQYILWWSFSIDRGSLQRLDISKLPEVPHHHHHH ADGGRSQQETRFVYESFLDQENLYIDKSATVLPSGILRLTNASEHQMGHAFHKKPLEFSSSGPLSFSTHFVCALVPKPRVEGGHGIAFVLSPSMDFTHAESTRYLGIFNASTSGSSSYHVLAVELDTIWNPDFKDIDHNHVGIDVNSPISVAIASASYFSDIKGSHERVDLLSGRPIQVWVDYEGTMLNVSIAPLKVQKPSRPLLSHPINLSKFFPNRSRLFVGFSASTGTAISDQYILWWSFSTRRGSLQGFDISKLPKVPHPHHHHHH

Contains a BamHI site.

Contains a NotI site.

§

The sequences derived from the cloning vector and the engineered C-terminal six-histidine tags are underlined.