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. 2016 Sep 22;72(Pt 10):782–787. doi: 10.1107/S2053230X16014278

Table 2. Crystallization of csI.9-ECD protein.

Method Vapour diffusion
Plate type Hanging drop
Temperature (K) 291
Protein concentration (mg ml−1) 5
Buffer composition of protein solution 20 mM Tris pH 8.0, 100 mM NaCl
Composition of reservoir solution 100 mM MES pH 6.5, 15% PEG 20 000
Volume and ratio of drop 4 µl drop, 1:1 protein:well solution
Volume of reservoir (µl) 400