Table 2. Crystallization of csI.9-ECD protein.
Method | Vapour diffusion |
Plate type | Hanging drop |
Temperature (K) | 291 |
Protein concentration (mg ml−1) | 5 |
Buffer composition of protein solution | 20 mM Tris pH 8.0, 100 mM NaCl |
Composition of reservoir solution | 100 mM MES pH 6.5, 15% PEG 20 000 |
Volume and ratio of drop | 4 µl drop, 1:1 protein:well solution |
Volume of reservoir (µl) | 400 |