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. 2016 Sep 27;72(Pt 10):795–798. doi: 10.1107/S2053230X16013406

Table 1. VTC1 construction and expression information.

Source organism A. thaliana
DNA source cDNA
Forward primer CATGCCATGGGCATGAAGGCACTCATTCTTGTTG
Reverse primer CCCAAGCTTCATCACTATCTCTGGCTTCAAGAT
Cloning vector pEASY-Blunt simple cloning vector
Expression vector pET-28a(+)
Expression host E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced MGMKALILVGGFGTRLRPLTLSFPKPLVDFANKPMILHQIEALKAVGVDEVVLAINYQPEVMLNFLKDFETKLEIKITCSQETEPLGTAGPLALARDKLLDGSGEPFFVLNSDVISEYPLKEMLEFHKSHGGEASIMVTKVDEPSKYGVVVMEESTGRVEKFVEKPKLYVGNKINAGIYLLNPSVLDKIELRPTSIEKETFPKIAAAQGLYAMVLPGFWMDIGQPRDYITGLRLYLDSLRKKSPAKLTSGPHIVGNVLVDETATIGEGCLIGPDVAIGPGCIVESGVRLSRCTVMRGVRIKKHACISSSIIGWHSTVGQWARIENMTILGEDVHVSDEIYSNGGVVLPHKEIKSNILKPEIVMKLAAALEHHHHHH