. 2016 Sep 27;72(Pt 10):795–798. doi: 10.1107/S2053230X16013406

Table 2. Crystallization of ligand-free VTC1 and co-crystallized VTC1.

	Ligand-free VTC1	Co-crystallized VTC1
Method	Sitting-drop vapour diffusion	Sitting-drop vapour diffusion
Plate type	48-well plates, Hampton Research	48-well plates, Hampton Research
Temperature (K)	289	289
Protein concentration (mg ml⁻¹)	7.5	7.5
Buffer composition of protein solution	20 mM Tris–HCl pH 7.5, 150 mM NaCl	20 mM Tris–HCl pH 7.5, 150 mM NaCl
Composition of reservoir solution	0.1 M bis-tris pH 6.5, 25%(w/v) PEG 3350, 0.2 M lithium sulfate	0.1 M magnesium formate, 15%(w/v) PEG 3350
Volume and ratio of drop	2 µl, 1:1	2 µl, 1:1
Volume of reservoir (µl)	200	200