Figure 1.

Tropomyosin proteins used in this study. (A) Predictive models of Tpm^Cdc8 (upper panel) and Cerulean3‐Tpm^Cdc8 (lower panel) dimers. (B) Coomassie blue‐stained SDS/PAGE analysis of purified wild‐type and Cerulean3‐tagged Tpm^Cdc8 proteins. (C) CD Spectra of purified acetylated wt (magenta), amino‐terminally Cerulean3‐tagged (black), and both acetylated (blue) and unacetylated (red) carboxyl‐terminally Cerulean3‐tagged Tpm^Cdc8 proteins. A CD spectrum of a fluorescent protein (cyan) is included for comparison. *Denotes amino‐terminally acetylated protein.