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Actin‐binding assays of FP Tpm fusions. SDS/PAGE gels of pellet and supernatant fractions from cosedimentation assays of (A) Cerulean3‐TpmCdc8, (B) TpmCdc8‐Cerulean3 and (C) Nt‐acetylated TpmCdc8‐Cerulean3. (D) Binding curve of the free TpmCdc8 concentration against the ratio of density of actin, for Cerulean3‐TpmCdc8, measured by densitometry of cosedimentation SDS/PAGE gels. Curves represent Hill equation lines of best fit.
