Table 1.

Summary of data presented

Protein	Expected Mass (kDa)	Determined mass (kDa)	T _m (°C)	Viscosity (cSt)	K _50% (μM)	Growth rate^a (∆OD₆₀₀ h⁻¹)	CAR ∅ constriction rate (μm·min⁻¹)^a	Cell length μm ( $\bar{x}$ ± SD)^a ^, ^b	Myo52 movements^a
Tpm^Cdc8	18 964	18 963.9	33.4	1.08	2.7	–	–	–	–
ace‐Tpm^Cdc8	19 006	19 006.4	35.4	1.22	0.6	0.08	0.088 ± 0.014	11.8 ± 3.0	+++
Cerulean3‐Tpm^Cdc8	47 002	47 001.6	35.5	1.17	0.69	0.063	0.09 ± 0.007	13.9 ± 4.3	+
Tpm^Cdc8‐Cerulean3	46 871	46 870.4	34.3	0.99	n.a.	–	0	24.2 ± 9.7	–
ace‐Tpm^Cdc8‐Cerulean3	46 913	46 912.4	35.7	1.02	n.a.	0	0	–	–

Determined in cdc8.110 cells at 36 °C.

GFP alone: 18.9 ± 4.3 μm.