Table 3.

Results of field (baited trapping) and eDNA sampling for rusty crayfish Orconectes rusticus in study lakes in Vilas County, WI, and Gogebic County, MI, USA, sampled during summer 2014. Catch per unit effort (CPUE) for O. rusticus is given as males (male) and both sexes combined (total), and both sexes combined (total) for the non‐target species Orconectes propinquus and Orconectes virilis, with standard deviations (SD). Positive detections of O. rusticus eDNA by qPCR are given as proportions of water samples, as well as the average eDNA copy number from samples by qPCR with standard deviations (SD). Lakes are ordered by sampling date and sequence (see Table 1)

Lake	Crayfish Catch per Unit Effort (CPUE; # per trap)
	Male (SD), Total O. rusticus (SD)	Total O. propinquus (SD), O. virilis (SD)	eDNA Detections	Average eDNA copy number (SD)
Tenderfoot	0·00 (0·00), 0·00 (0·00)	0·15 (0·37), 0·00 (0·00)	1/10 (0·10)	0·061 (0·193)
Van Vliet	0·08 (0·00), 0·17 (0·40)	0·00 (0·00), 0·58 (0·50)	1/10 (0·10)	0·003 (0·008)
Clear	0·11 (0·32), 0·22 (0·54)	0·00 (0·00), 0·00 (0·00)	1/10 (0·10)	0·081 (0·257)
Spider	1·52 (1·77), 1·83 (2·07)	0·00 (0·00), 0·04 (0·20)	6/10 (0·60)	7·089 (18·686)
Little Star	1·71 (1·99), 1·83 (2·12)	0·00 (0·00), 0·00 (0·00)	9/10 (0·90)	2·750 (3·910)
Boulder	5·67 (6·19), 7·29 (7·52)	0·00 (0·00), 0·13 (0·61)	8/10 (0·80)	3·809 (5·614)
South Turtle	7·83 (8·96), 8·25 (9·24)	0·17 (0·38), 0·13 (0·45)	9/10 (0·90)	330·231 (1036·274)
Big	10·80 (11·81), 13·35 (13·58)	0·00 (0·00), 0·00 (0·00)	7/10 (0·70)	2·896 (5·030)
Papoose	10·42 (6·88), 11·42 (7·33)	0·00 (0·00), 0·00 (0·00)	8/8 (1·00)	2·540 (2·488)
Jute	0·00 (0·00), 0·00 (0·00)	0·00 (0·00), 0·00 (0·00)	0/10 (0·00)	0·000 (0·000)
Allequash	0·00 (0·00), 0·00 (0·00)	0·00 (0·00), 0·00 (0·00)	1/10 (0·10)	0·086 (2·640)
Little John	8·49 (7·02), 8·86 (7·03)	0·00 (0·00), 0·00 (0·00)	7/10 (0·70)	1·604 (2·643)