Figure 4. Reduced adipogenic differentiation potential of Tks4^−/− BM-MSCs.

In vitro adipogenesis of MSCs isolated from bone marrow of wild type and Tks4^−/− mice. (a) Representative Oil red O-stained cultures and (b) the quantification of Oil Red O content (n = 8) following differentiation for 7 days. (c) Time course of Tks4 protein expression in wild type MSCs during adipogenic differentiation. Cell lysates were prepared at various time points and Western blot analyses were performed with anti-Tks4 antibody. Tubulin was used to control equal loading. (d) Time course of adiponectin and PPAR_ϒ expression during in vitro adipocyte differentiation of wild type and Tks4^−/− BM-MSCs. Cell lysates were prepared at various time points and Western blot analysis were performed. Tubulin was used to control equal loading. Gels were run simultaneously under the same experimental conditions. The adipo-differentiated wild type (e) and Tks4^−/− MSCs (f) were subjected to a TaqMan array for mouse lipid-regulated genes and gene expression profile was analyzed. The mRNA levels measured for day 0 of differentiation (control) were set to 1. The mRNA levels measured for day 4 of differentiation are calculated as n-fold differences relative to the control (day 0) samples. The relative expression levels of each gene are shown. (e,f) Indicates genes at least 2-fold up- or downregulation²⁹.