Table 2.
Antibodies used in the study
| Primary antibodies | Catalogue no. | Company | Host | Clonality | Dilution |
|---|---|---|---|---|---|
| Antigen | |||||
| Neurofilament (NF, 2F11) | M0762 | Dako | Mouse | Monoclonal | 1:50–1:100 |
| Ionized calcium‐binding adapter molecule 1 (IBA1) | 019‐19741 | Wako | Rabbit | Polyclonal | 1:50 |
| α‐synuclein (α‐syn, 42) | 610787 | Becton Dickson (BD) | Mouse | Monoclonal | 1:50 |
| Tyrosine hydroxylase (TH) | AB152 | Millipore | Rabbit | Polyclonal | 1:50–1:100 |
| Secondary antibodies | Catalogue no. | Company | Host | Fluorophore conjugates | Dilution |
|---|---|---|---|---|---|
| Name | |||||
| AlexaFluor anti‐goat | A‐11055 | Invitrogen | Donkey | 488 IgG (H+L) | 1:50 |
| AlexaFluor anti‐goat | A‐11057 | Invitrogen | Donkey | 568 IgG (H+L) | 1:50 |
| AlexaFluor anti‐rabbit | A10042 | Invitrogen | Donkey | 568 IgG (H+L) | 1:50 |
| AlexaFluor anti‐mouse | A‐21202 | Invitrogen | Donkey | 488 IgG (H+L) | 1:50 |
| AlexaFluor anti‐rabbit | A‐11011 | Invitrogen | Goat | 568 IgG (H+L) | 1:50 |
| AlexaFluor anti‐mouse | A‐11001 | Invitrogen | Goat | 488 IgG (H+L) | 1:50 |