Table 4.
Comparison of tissue clearing speed between rat and human brain tissue blocks (3‐mm thick)
| Rat | Human (fresh) | Human (formalin‐fixed) | |
|---|---|---|---|
| Post‐perfusion immersion fixation | 2 days | 5–7 days | 7–10 days |
| Washing | 2 days | 2 days | 2 days |
| Passive thermal clearing |
5–10 days (cerebral coronal slices) ~18 days (brainstem) |
7–10 days (subcortical blocks) ~40 days (cortical blocks) |
~60 days (cortical blocks) Clearing not achieved for brainstem |
| Washing | 2 days | 2 days | 2 days |
| Total clearing time from dissection | ~11–24 days | ~15–51 days | ~70 days |