Table 5.
Current studies with CLARITY involving the use of human brain tissues
| Reference | Cases | Sample | Tissue type | Thickness (μm) | Clearing time | Immunostaining time | Antibodies used | Refractive index matching medium |
|---|---|---|---|---|---|---|---|---|
| Chung et al. (2013) 13 | 7‐year old male with autism; 10‐year old male control | Frontal cortex | Formalin‐fixed | 500 | 1 day (active clearing) | 3 days | MBP, TH, PV, NF | FocusClear |
| Ando et al. (2014) 14 |
5 Alzheimer's disease 2 controls |
Frontal cortex | Formalin‐fixed | 500 | 2 weeks (37°C, passive clearing) | Not reported | Abeta, tau, NF | ScaleA2 |
| Costantini et al. (2015) 15 | 1 child with hemi‐megalencephaly | Cortex | Formalin‐fixed | 2000 | 2 weeks (37°C, passive clearing) | 6 days | PV, GFAP | 47% TDE |
GFAP, Glial fibrillary acidic protein; MBP, myelin basic protein; NF, neurofilament; PV, Parvalbumin; TDE, 2,2′‐Thiodiethanol; TH, tyrosine hydroxylase.