Figure 5.

Plasminogen activator inhibitor‐1 (PAI‐1) promotes neutrophil recruitment during Yersinia pestis infection. C57BL/6 or C57BL/6 PAI‐1 ^−/− mice were inoculated with phosphate‐buffered saline (mock), 10⁴ colony‐forming units (CFUs) of Y. pestis, or 10⁴ CFUs of Y. pestis ∆pla, and after 48 h bronchoalveolar lavage was performed to recover immune cells. (A) The total numbers of cells recruited to the lungs were determined by counting on a hemacytometer. (B–G) Flow cytometry was performed to determine the absolute numbers of (B) neutrophils, (C) alveolar macrophages, (D) CD11b hi macrophages, (E) monocytes, (F) CD11b hi dendritic cells (DCs) and (G) CD11b lo DCs recruited to the lungs. Data from two or three independent experiments are combined (n = 10–15 for each group); error bars represent standard errors of the mean. One‐way anova with Bonferroni's multiple comparison test was used to determine significance (*P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001). NS, not significant.