Table 2.

Structure of synthetic compounds and their cholinesterase activities over acetylcholinesterase (AChE) from Electrophorus electricus and butyrylcholinesterase (BChE) from equine serum

Comp.	R	N‐alkyl	AChE (μ m)a	BChE (μ m)a	SIb
4aa	H	CH2=CHCH2	211.72 ± 0.02	456.95 ± 0.03	2.16
4ab	CH3	CH2=CHCH2	75.17 ± 0.01	31.66 ± 0.01	0.42
4ac	OCH3	CH2=CHCH2	75.10 ± 0.01	62.23 ± 0.02	0.83
4ad	Cl	CH2=CHCH2	293.52 ± 0.02	62.26 ± 0.08	0.21
4ae	CH2CH3	CH2=CHCH2	173.96 ± 0.02	25.58 ± 0.02	0.15
4af	F	CH2=CHCH2	72.91 ± 0.01	135.29 ± 0.02	1.86
4ag	Br	CH2=CHCH2	168.03 ± 0.06	29.08 ± 0.03	0.17
4ba	H	HC≡CCH2	421.30 ± 0.03	896.70 ± 0.03	2.13
4bb	CH3	HC≡CCH2	259.63 ± 0.01	455.59 ± 0.01	1.75
4bc	OCH3	HC≡CCH2	412.69 ± 0.01	1206.84 ± 0.01	2.92
4bd	Cl	HC≡CCH2	392.91 ± 0.04	1327.55 ± 0.17	3.38
4be	CH2CH3	HC≡CCH2	443.66 ± 0.01	1976.73 ± 0.09	4.46
4bf	F	HC≡CCH2	375.61 ± 0.03	894.37 ± 0.03	2.38
4bg	Br	HC≡CCH2	661.38 ± 0.09	894.77 ± 0.06	1.35
Galantamine	–	–	0.54 ± 0.7	8.80 ± 0.5	16.29

^aValues are the average from three independent experiments.

^bSelectivity for AChE is defined as IC₅₀ (BChE)/IC₅₀ (AChE).