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. 2015 Mar 11;9(5):619–631. doi: 10.1002/term.2003

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© 2015 The Authors. Journal of Tissue Engineering and Regenerative Medicine Published by John Wiley & Sons Ltd.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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qRT–PCR analyses of the expression levels for selected genes associated with the osteoblastic differentiation process of BM‐MSCs cultured in standard cell culture medium (Std) and osteogenic cell culture medium (Ost) at 1, 3 and 6 days of exposure to 20 mg Sr0, Sr50 and Sr100 bioactive glass powders: (A) Bmp2; (B) Runx2; (C) Alpl; (D) Col1a1; (E) Spp1; and (F) Bglap. All fold changes were normalized to the values of the expression of each gene in the control samples (i.e. BM‐MSCs cultured in standard cell culture medium and not exposed to any amount of bioactive glass powder)