Fig. 6.

Levels of circulating antigen-specific IgG1 and IgG2c antibodies in infected WT and TLR^−/− mice. WT and TLR^−/− mice were infected with 10⁵  L. major promastigotes (a, b), L. mexicana promastigotes (c, d) or L. mexicana amastigotes (e, f). At the end of each experiment (week 10 or 18 for L. major infection, week 14 for L. mexicana infections), blood was collected from mice via cardiac puncture and the plasma was collected. Levels of antigen-specific antibodies were calculated using antibody ELISA with either L. major FTAg (a, b) or L. mexicana WMAg (c, d, e, f) as the capture antigen. Quantities are displayed as OD values for individual mice, as well as the median value for each group. Groups were compared using a Mann-Whitney U test P < 0.05 (*) and P < 0.01 (**) were considered to indicate significant differences