Fig. 2.

a 2D-DIGE dye overlay gel image (see Table 4 for sample composition). The first dimension was performed using a 17 cm IPG strip, and the second dimension using a 12 % linear acrylamide gel. Spots that showed changes in abundance are numbered and encircled; the position numbers correspond to the spot numbers listed in Table 1. The relative changes in normalized spot volume of protein amount were assessed by biological variation (BVA) across all the DIGE experiments. b Classification of the identified proteins according to their biological function, using the NCBI, Uniprot and KEEG databases and bibliographic search