Table 2.
Prediction of the protein kinases targeting the phosphorylated sites identified by LC–MS/MS in protein Pc22g05690
| Site | Kinase | Score | Phosphopeptides identified (LC–MS/MS) |
|---|---|---|---|
| T-279 | PKA | 0.64 | (R)KRpTSIFGTLLGK(K) |
| S-280 | PKA | 0.64 | (R)KRTpSIFGTLLGK(K) (K)RTpSIFGTLLGK(K) (R)TpSIFGTLLGK(K) |
| T-284 | PKC | 0.85 | (R)KRTSIFGpTLLGK(K) |
| S-323 | – | – | (K)AAEPTTESpSAEAPAPVAAETAETAAAPTK(A) |
| T-351 | CKII | 0.55 | (K)AAEPTAEpTPAETTEAAKEEAK(D) |
| S-377 | PKA | 0.78 | (K)RApSIFGNFFQK(V) |
| S-388 | p38MAPK | 0.56 | (K)VApSPSQEK(S) (K)VApSPSQEKSEK(E) |
| S-462 | PKA | 0.8 | (K)RRTpSFFGNLGMK(K) |
| S-477 | CKII | 0.66 | (K)EKKADpSDNEVTDGEAK(E) (K)ADpSDNEVTDGEAK(E) (K)KADpSDNEVpTDGEAKETK(A) |
| T-482 | CKII | 0.72 | (K)KADSDNEVpTDGEAKETK(A) (K)KADpSDNEVpTDGEAKETK(A) |
| S-502 | PKA | 0.69 | (R)KPpSKAVKLDKEEVAAAETEAK(A) |
The analysis was performed with the software NetPhosK 1.0 [55]