Fig 6. Serum cytokine profiles and percentage of splenic neutrophils following B. pertussis challenge in unprotected and protected mice.

(A) The serum concentrations of 33 cytokines were analyzed before and after a B. pertussis challenge in unprotected (lighter blue bars) and protected (dark blue and red bars) mice, as indicated. Concentrations of CXCL13, CCL11, CXCL1, G-CSF, IL-6, CXCL10, and IL-13 serum were significantly altered and represented as mean concentrations of individual values (n = 3). Significant values were calculated by one-way ANOVA with multiple comparison compared to the pre-challenge level (D0) of unprotected mice or protected mice (* = p<0.05, ** = p<0.01, and *** = p<0.001). (B) The percentage of Gr1⁺ cells (neutrophils) was determined over time in the spleen of unprotected and protected mice by using Flow cytometry (* = p<0.05).