Skip to main content
. Author manuscript; available in PMC: 2016 Oct 6.
Published in final edited form as: DNA Repair (Amst). 2014 Jan 25;20:41–48. doi: 10.1016/j.dnarep.2013.10.012

Fig. 4.

Fig. 4

UvrAB complex with UvrB-Qdot. A hemagglutinin (HA) epitope tag (YPYD-VPDYA) was engineered on to the N-terminus of UvrB to which was conjugated a mouse monoclonal antibody. A goat antimouse coated Qdot was bound to the UvrB-HA-Ab to make an “antibody” sandwich. This UvrB-HA-Ab-Ab-Qdot complex was mixed with UvrA and a 517 bp fragment prepared by PCR and containing a nick 40% from one end. This AFM image shows the transient complex of UvrA loading UvrB-HA-Ab-Ab-Qdot at the site of a nick, only 6% of the total complexes found on DNA had both UvrB and UvrA bound at the site of the nick.

Adapted from [44] with permission.