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. 2016 Oct 6;11(10):e0163379. doi: 10.1371/journal.pone.0163379

Fig 6. Expression of selected P. xanthii CSEP genes in a time-course experiment.

Fig 6

Total RNA was isolated from zucchini cotyledons infected with P. xanthii at different time points, and the relative expression of five putative effector-genes (CSEP01, CSEP02, CSEP05, CSEP021 and a homologue to Scp160) was analysed by quantitative RT-PCR. Transcript abundance was normalised to the endogenous control β-tubulin gene PxTUB2 (KC333362). Relative expression of each gene was calibrated to the sample 8 h post inoculation. Points represent the mean ± standard deviation of three technical replicates from two cDNA samples obtained from two different zucchini cotyledons.